Inhibition of Anion Transport in Corn Root Protoplasts
نویسندگان
چکیده
منابع مشابه
Sugar transport in isolated corn root protoplasts.
Isolated corn (Zea mays L.) root protoplasts were used to study sucrose and hexose uptake. It is found that glucose was preferentially taken up by the protoplasts over sucrose and other hexoses. Glucose uptake showed a biphasic dependence on external glucose concentration with saturable (K(m) of 7 millimolar) and linear components. In contrast, sucrose uptake only showed a linear kinetic curve....
متن کاملCorn Root Protoplasts: ISOLATION AND GENERAL CHARACTERIZATION OF ION TRANSPORT .
A method was developed for the large scale and rapid isolation of intact viable corn root protoplasts. Pure and metabolically active protoplasts were collected using a flotation technique. Vital staining tests, light and electron microscopy, and measurements of basic metabolic processes indicated that the isolated protoplasts were metabolically active, and that the plasmalemma and other organel...
متن کاملFurther Characterization on the Transport Property of Plasmalemma NADH Oxidation System in Isolated Corn Root Protoplasts.
Recent experiments show that exogenous NADH increases the O(2) consumption and uptake of inorganic ions into isolated corn (Zea mays L. Pioneer Hybrid 3320) root protoplasts (Lin 1982, Proc Natl Acad Sci USA 79: 3773-3776). A mild treatment of protoplasts with trypsin released most of the NADH oxidation system from the plasmalemma (Lin 1982 Plant Physiol 70: 326-328). Further studies on this sy...
متن کاملSalinity affects intracellular calcium in corn root protoplasts.
Previous work with the fluorescent Ca probe chlorotetracycline (CTC) showed that salinity displaces Ca from membranes of root cells. Using a variety of indirect approaches, we studied whether salinity displaces Ca from the cell surface or from internal membranes of corn (Zea mays L. cv Pioneer 3377) root protoplasts. Preloading the cells with supplemental Ca counteracted subsequent NaCl effects...
متن کاملSalinity reduces membrane-associated calcium in corn root protoplasts.
Calcium is an important factor in the ability of plants to resist salt stress, possibly because of its role in maintaining membrane integrity. We studied the effects of NaCl stress on membrane-associated Ca in corn root protoplasts (Zea mays L. cv Pioneer 3377) using the fluorescent Ca probe chlorotetracycline (CTC). Protoplasts were isolated from the cortex of primary roots of corn seedlings (...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1981
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.68.2.435